Phytochemical screening, antimicrobial and antioxidant efficacy of different extracts of Rumex dentatus L. - A locally used medicinal herb of Kashmir Himalaya

Content Provider	Europe PMC
Author	Nisa, Humeera Kamili, Azra N. Bandh, Suhaib A. Amin, Shajr-ul Lone, Bashir A. Parray, Javaid A.
Copyright Year	2013
Abstract	Objective To elucidate the antimicrobial and antioxidant activities of Rumex dentatus L. (R. dentatus) along with its phytochemical analysis. Methods Agar disk diffusion method for antimicrobial activity and DPPH, riboflavin photo-oxidation, deoxyribose and lipid peroxidation assay for antioxidant activity. Results The antimicrobial and antioxidant activities of different concentrations of five R. dentatus extracts were tested against different clinical bacterial strains (Shigella flexneri, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhimurium) and fungal strains (Aspergillus versicolor, Aspergillus flavus, Accremonium spp., Penicillium dimorphosporum, Candida albicans, Candida parapsilosis, and Candida kruesie). Among all extracts, the butanol extract showed strong antibacterial activity against Klebsiella pneumoniae (inhibition zone diameter of 20 mm) and aqueous extract showed no activity against any of the bacterial strains. While as in case of the fungal strains, the maximum antifungal activity was observed against Aspergillus flavus by aqueous extract. The antioxidant activity revealed that the extracts exhibited scavenging effect in concentration-dependent manner on superoxide anion radicals and hydroxyl radicals. The phytochemical tests carried out with the crude extracts of R. dentatus showed the presence of flavonoids, terpenoids, alkaloids, saponins, tannins, anthraquinones and cardiac glycosides in it. The total phenolic content of these extracts was estimated quantitatively from standard calibration curve of gallic acid and it varied from 145 µg/mg in butanol extract to 45 µg/mg in petroleum ether extract. Conclusions It can be concluded that the plant has got a broad spectrum antimicrobial and antioxidant activity and could be used as a potential alternative for treating various diseases.
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ISSN	22221808
Volume Number	3
PubMed Central reference number	PMC4027333
Issue Number	6
PubMed reference number	PMC4027333
Journal	Asian Pacific Journal of Tropical Disease [Asian Pac J Trop Dis]
e-ISSN	22221808
DOI	10.1016/s2222-1808(13)60097-3
Language	English
Publisher	Asian Pacific Tropical Medicine Press
Publisher Date	2013-12-01
Access Restriction	Open
Rights License	© 2013 by the Asian Pacific Journal of Tropical Disease. All rights reserved.
Subject Keyword	Antimicrobial activity Antioxidant activity Rumex dentatus Medicinal herb
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)