NDLI: Effects of Shiga toxin type 2 on maternal and fetal status in rats in the early stage of pregnancy.

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Effects of Shiga toxin type 2 on maternal and fetal status in rats in the early stage of pregnancy.

Content Provider	Europe PMC
Author	Sacerdoti, Flavia Amaral, María M. Zotta, Elsa Franchi, Ana M. Ibarra, Cristina
Copyright Year	2014
Abstract	Shiga toxin type 2 (Stx2), a toxin secreted by Shiga toxin-producing Escherichia coli (STEC), could be one of the causes of maternal and fetal morbimortality not yet investigated. In this study, we examined the effects of Stx2 in rats in the early stage of pregnancy. Sprague-Dawley pregnant rats were intraperitoneally (i.p.) injected with sublethal doses of Stx2, 0.25 and 0.5 ng Stx2/g of body weight (bwt), at day 8 of gestation (early postimplantation period of gestation). Maternal weight loss and food and water intake were analyzed after Stx2 injection. Another group of rats were euthanized and uteri were collected at different times to evaluate fetal status. Immunolocalization of Stx2 in uterus and maternal kidneys was analyzed by immunohistochemistry. The presence of Stx2 receptor (globotriaosylceramide, Gb3) in the uteroplacental unit was observed by thin layer chromatography (TLC). Sublethal doses of Stx2 in rats caused maternal weight loss and pregnancy loss. Stx2 and Gb3 receptor were localized in decidual tissues. Stx2 was also immunolocalized in renal tissues. Our results demonstrate that Stx2 leads to pregnancy loss and maternal morbidity in rats in the early stage of pregnancy. This study highlights the possibility of human pregnancy loss and maternal morbidity mediated by Stx2.
ISSN	23146133
Journal	Biomed Research International
Volume Number	2014
PubMed Central reference number	PMC4026949
PubMed reference number	25157355
e-ISSN	23146141
DOI	10.1155/2014/384645
Language	English
Publisher	Hindawi
Publisher Date	2014-05-05
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2014 Flavia Sacerdoti et al.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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