Zinc and low-dose of cadmium protect sertoli cells against toxic-dose of cadmium: The role of metallothionein.

Content Provider	Europe PMC
Author	Kheradmand, Fatemeh Nourmohammadi, Issa Ahmadi-Faghih, Mohamad Amin Firoozrai, Mohsen Modarressi, Mohammad Hossein
Abstract	Background: The impact of cadmium (Cd) on male infertility may be related to the interaction with metal-binding proteins known as metallothioneins (Mts). Trace elements like zinc (Zn) have protective effects on testicular damage induced by Cd.Objective: We determined the effect of Zn and low-dose Cd pre-treatment on the expression of Mt1 and Mt2 genes on testicular Sertoli cells.Materials and Methods: The cultured TM4 mouse sertoli cells were treated with 50 μM ZnSO4 (Zn pre-treated group; ZnPG), 2 μM CdCl2 (Cd pre-treated group; CdPG), or distilled water (DW pre-treated group; DWPG). After 18 hour, all of these groups were exposed to 100 μM CdCl2 for different periods of time (1, 2, 3, and 6 hours). There was also a control group for all three groups, which was treated only with distilled water (without Cd or Zn pre-treatment). Cellular viability, Zn and Cd concentrations and gene expression were assessed by MTT, atomic absorption spectrometry and real time PCR methods, respectively.Results: The expression of Mt1 and Mt2 genes in ZnPG, CdPG, and DWPG was greater than the control group (p=0.02 and p=0.01, respectively). Cd concentrations in CdPG and DWPG were greater than the control group (p=0.00). Expression of both genes in ZnPG and CdPG increased after 3 hours of treatment and Cd concentration decreased simultaneously, which was more obvious in ZnPG.Conclusion: Zn and short term low-dose Cd pre-treatment might reduce the adverse effects of Cd by increasing expression of Mts genes in Sertoli cells. The protective effect of Zn was stronger than Cd.
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ISSN	16806433
Journal	Iranian Journal of Reproductive Medicine [Iran J Reprod Med]
Volume Number	11
PubMed Central reference number	PMC3941315
Issue Number	6
PubMed reference number	24639783
e-ISSN	20082177
Language	English
Publisher	Research and Clinical Center for Infertility
Publisher Date	2013-06-01
Publisher Place	Yazd, Iran
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Subject Keyword	Cadmium Gene expression Metallothionein Sertoli cells Zinc
Content Type	Text
Resource Type	Article
Subject	Reproductive Medicine Obstetrics and Gynecology