Cytotoxic and Genotoxic effects of Arsenic and Lead on Human Adipose Derived Mesenchymal Stem Cells (AMSCs).

Content Provider	Europe PMC
Author	Shakoori, AR Ahmad, A
Copyright Year	2013
Abstract	Arsenic and lead, known to have genotoxic and mutagenic effects, are ubiquitously distributed in the environment. The presence of arsenic in drinking water has been a serious health problem in many countries. Human exposure to these metals has also increased due to rapid industrialization and their use in formulation of many products. Liposuction material is a rich source of stem cells. In the present study cytotoxic and genotoxic effects of these metals were tested on adipose derived mesenchymal stem cells (AMSCs). Cells were exposed to 1-10 μg/ml and 10-100 μg/ml concentration of arsenic and lead, respectively, for 6, 12, 24 and 48 h. The cytotoxic effects were measured by neutral red uptake assay, while the genotoxic effects were tested by comet assay. The growth of cells decreased with increasing concentration and the duration of exposure to arsenic. Even the morphology of cells was changed; they became round at 10 μg /ml of arsenic. The cell growth was also decreased after exposure to lead, though it proved to be less toxic when cells were exposed for longer duration. The cell morphology remained unchanged. DNA damage was observed in the metal treated cells. Different parameters of comet assay were investigated for control and treated cells which indicated more DNA damage in arsenic treated cells compared to that of lead. Intact nuclei were observed in control cells. Present study clearly demonstrates that both arsenic and lead have cytotoxic and genotoxic effects on AMSCs, though arsenic compared to lead has more deleterious effects on AMSCs.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3908312&blobtype=pdf
Page Count	8
Volume Number	9
PubMed Central reference number	PMC3908312
Issue Number	2
PubMed reference number	24693207
Journal	Journal of Stem Cells & Regenerative Medicine [J Stem Cells Regen Med]
e-ISSN	09737154
Language	English
Publisher	Journal of Stem Cells and Regenerative Medicine
Publisher Date	2013-10-15
Access Restriction	Open
Rights License	This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2013 Journal of Stem Cells and Regenerative Medicine
Subject Keyword	Anti-proliferative activity cytotoxic effect comet assay heavy metals genotoxic effects
Content Type	Text
Resource Type	Article
Subject	Biochemistry Cell Biology Molecular Biology Biotechnology