Inhibition of p65 Nuclear Translocation by Baicalein.

Content Provider	Europe PMC
Author	Seo, Min Bum Lee, Seog Ki Jeon, Young Jin Im, Jin Su
Copyright Year	2011
Abstract	We demonstrate that baicalein, a bioactive flavonoid originally isolated from Scutellaria baicalensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of peritoneal macrophages and RAW 264.7 cells with baicalein inhibited LPS-stimulated nitric oxide production in a dose-related manner. Immunohistochemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression in RAW 264.7 cells. Immunostaining of p65, EMSA, and reporter gene assay showed that baicalein inhibited NF-κB nuclear translocation, DNA binding, and transcriptional activation, respectively. Collectively, these series of experiments indicate that baicalein inhibits iNOS gene expression by blocking NF-κB nuclear translocation. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of baicalein on iNOS suggest that baicalein may represent a useful anti-inflammatory agent.
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ISSN	19768257
Journal	Toxicological Research [Toxicol Res]
Volume Number	27
PubMed Central reference number	PMC3834365
Issue Number	2
PubMed reference number	24278554
DOI	10.5487/tr.2011.27.2.071
Language	English
Publisher	The Korean Society of Toxicology
Publisher Date	2011-06-01
Access Restriction	Open
Rights License	Copyright ©2011, The Korean Society of Toxicology
Subject Keyword	Baicalein Macrophages iNOS NF-κB
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Toxicology