NDLI: Identification of a cancer stem cell-like side population in the HeLa human cervical carcinoma cell line.

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Identification of a cancer stem cell-like side population in the HeLa human cervical carcinoma cell line.

Content Provider	Europe PMC
Author	WANG, KEFANG ZENG, JIANFANG LUO, LIJING YANG, JIAXIN CHEN, JIE LI, BIN SHEN, KENG
Copyright Year	2013
Abstract	The present study aimed to identify the stem cell characteristics of side population (SP) cells sorted from the widely-used HeLa human cervical carcinoma cell line. The SP cells were sorted from the HeLa cell line using fluorescence-activating cell sorting (FACS). Stem cell characteristics of the SP cells, including proliferation, self-renewal, differentiation and the ability to form xenografts, were investigated in vitro and in vivo. The SP cells demonstrated strong tumorigenesis following in vivo transplantation into five to six-week-old female Balb/c mice. The SP cells were observed to be more resistant to chemotherapy and radiotherapy compared with non-side population (NSP) cells. A higher expression of CD133 was observed in the SP cells compared with the NSP cells following FACS. The results demonstrated that the SP cells from the HeLa human cervical carcinoma cell line exhibit stem cell characteristics in vitro and also have a strong ability to form tumors in vivo. The cell surface marker CD133 may serve as a potential molecular marker for the identification of cervical cancer stem cells (CSCs).
ISSN	17921074
Journal	Oncology Letters
Volume Number	6
PubMed Central reference number	PMC3833864
Issue Number	6
PubMed reference number	24260061
e-ISSN	17921082
DOI	10.3892/ol.2013.1607
Language	English
Publisher	D.A. Spandidos
Publisher Date	2013-10-07
Access Restriction	Open
Rights License	This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. Copyright © 2013, Spandidos Publications
Subject Keyword	cervical carcinoma HeLa cell line side population cells cancer stem cells CD133
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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