NDLI: Effect of Jatropha curcas peptide fractions on the angiotensin I-converting enzyme inhibitory activity.

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Effect of Jatropha curcas peptide fractions on the angiotensin I-converting enzyme inhibitory activity.

Content Provider	Europe PMC
Author	Segura-Campos, Maira R. Peralta-González, Fanny Castellanos-Ruelas, Arturo Chel-Guerrero, Luis A. Betancur-Ancona, David A.
Copyright Year	2013
Abstract	Hypertension is one of the most common worldwide diseases in humans. Angiotensin I-converting enzyme (ACE) plays an important role in regulating blood pressure and hypertension. An evaluation was done on the effect of Alcalase hydrolysis of defatted Jatropha curcas kernel meal on ACE inhibitory activity in the resulting hydrolysate and its purified fractions. Alcalase exhibited broad specificity and produced a protein hydrolysate with a 21.35% degree of hydrolysis and 34.87% ACE inhibition. Ultrafiltration of the hydrolysate produced peptide fractions with increased biological activity (24.46–61.41%). Hydrophobic residues contributed substantially to the peptides' inhibitory potency. The 5–10 and <1 kDa fractions were selected for further fractionation by gel filtration chromatography. ACE inhibitory activity (%) ranged from 22.66 to 45.96% with the 5–10 kDa ultrafiltered fraction and from 36.91 to 55.83% with the <1 kDa ultrafiltered fraction. The highest ACE inhibitory activity was observed in F2 (IC50 = 6.7 μg/mL) from the 5–10 kDa fraction and F1 (IC50 = 4.78 μg/mL) from the <1 kDa fraction. ACE inhibitory fractions from Jatropha kernel have potential applications in alternative hypertension therapies, adding a new application for the Jatropha plant protein fraction and improving the financial viability and sustainability of a Jatropha-based biodiesel industry.
ISSN	23146133
Journal	Biomed Research International
Volume Number	2013
PubMed Central reference number	PMC3810520
PubMed reference number	24224169
e-ISSN	23146141
DOI	10.1155/2013/541947
Language	English
Publisher	Hindawi
Publisher Date	2013-10-10
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2013 Maira R. Segura-Campos et al.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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