NDLI: Study on thermodynamics and adsorption kinetics of purified endoglucanase (CMCase) from Penicillium notatum NCIM NO-923 produced under mixed solid-state fermentation of waste cabbage and bagasse.

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Study on thermodynamics and adsorption kinetics of purified endoglucanase (CMCase) from Penicillium notatum NCIM NO-923 produced under mixed solid-state fermentation of waste cabbage and bagasse.

Content Provider	Europe PMC
Author	Das, Arpan Ghosh, Uma Mohapatra, Pradeep Kumar Das Pati, Bikas Ranjan Mondal, Keshab Chandra
Copyright Year	2012
Abstract	In the current study, one thermostable endoglucanase was purified from Penicillium notatum NCIM NO-923 through mixed solid state fermentation of waste cabbage and bagasse. The molecular weight of the purified enzyme was 55kDa as determined by SDS polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme had low activation energy (Ea) of 36.39KJ mol-1 for carboxymethyl cellulose hydrolysis and the enthalpy and entropy for irreversible inactivation was 87 kJ mol −1 and 59.3 J mol −1 K−1 respectively. The enzyme was quite thermostable with a Tm value of 62.2°C. The pKa1 and pKa2 of ionizable groups of the active sites were 2.5 and 5.3 respectively. Apparent Km, Vmax and Kcat of the enzyme were found to be 5.2 mg mL-1, 80 U/gds and 322.4 sec-1 respectively. The enzyme showed about 1.4 fold increased activity in presence of 10mM MgSO4. Adsorption of endoglucanase on Avicel at wide pH range was studied at different temperatures. Langmuir type adsorption isotherm at 10°C showed maximum adsorption strength of enzyme at pH 3.0, which was in a range of optimum pH of the enzyme.
ISSN	15178382
Journal	Brazilian Journal of Microbiology
Volume Number	43
PubMed Central reference number	PMC3768859
Issue Number	3
PubMed reference number	24031934
e-ISSN	16784405
DOI	10.1590/S1517-838220120003000037
Language	English
Publisher	Sociedade Brasileira de Microbiologia
Publisher Date	2012-06-01
Access Restriction	Open
Rights License	All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License © Sociedade Brasileira de Microbiologia
Subject Keyword	Endoglucanase Langmuir adsorption isotherm Penicillium notatum Solid state fermentation
Content Type	Text
Resource Type	Article
Subject	Microbiology

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