NDLI: Crystallization and preliminary X-ray diffraction analysis of a thermostable GDSL-family esterase, EstL5, from Geobacillus thermodenitrificans T2.

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Crystallization and preliminary X-ray diffraction analysis of a thermostable GDSL-family esterase, EstL5, from Geobacillus thermodenitrificans T2.

Content Provider	Europe PMC
Author	Yang, Zhenxing Zhang, Yong Qiu, Rui Huang, Jing Ji, Chaoneng
Copyright Year	2013
Description	As a thermostable esterase with cold adaptation, EstL5 might be of significant industrial interest and value in scientific research. In order to provide new insights into the structure–function relationship of EstL5 and to help better understand the biochemical data, a project to determine the three-dimensional structure of this enzyme was initiated. The novel thermostable esterase EstL5 belonging to the GDSL family exhibits a unique cold-adaptation feature at low temperatures. To better understand its biochemical and enzymatic properties, recombinant EstL5 protein was purified and crystallized using the vapour-diffusion method. The EstL5 crystals diffracted X-rays to 2.79 Å resolution using a synchrotron-radiation source, belonged to the tetragonal space group P41212 or P43212, with unit-cell parameters a = b = 101.51, c = 124.22 Å, and are expected to contain two molecules in each asymmetric unit. To obtain initial phases, selenomethionyl-substituted protein was overproduced. Purified SeMet-labelled EstL5 protein was crystallized and formed crystals that diffracted to a resolution of 3.0 Å.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3702323&blobtype=pdf
Volume Number	69
PubMed Central reference number	PMC3702323
Issue Number	Pt 7
Issue Number	pt 7
PubMed reference number	23832206
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun]
e-ISSN	17443091
DOI	10.1107/s174430911301498x
Language	English
Publisher	International Union of Crystallography
Publisher Date	2013-06-28
Access Restriction	Open
Rights License	© International Union of Crystallography 2013
Subject Keyword	EstL5 thermostability GDSL esterases Geobacillus thermodenitrificans
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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