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Expression of CagL from Helicobacter pylori and Preliminary Study of its Biological Function.
| Content Provider | Europe PMC |
|---|---|
| Author | Wang, Hua Huang, Shiteng Zhao, Jianzhong Han, Jun Guan, Xianwei Shao, Shihe |
| Abstract | Helicobacter pylori (H. pylori) is a highly successful human-specific gastric pathogen, infecting over half the world’s population. Virulent H. pylori isolates harbour the cytotoxin-associated genes pathogenicity island (cag-PAI), the majority of which have no known function. In this study, we used cell infection assay and reverse transcriptase PCR, identified that CagL recombinant protein, one of the cag-PAI proteins, induced GES-1 cells to express cytokine IL-8. Then we performed western blot and translocation assay. Our result showed CagL polyclonal antibody counteracted translocation of CagA. This will provide a foundation for the further studies on its biological function. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3587514&blobtype=pdf |
| ISSN | 00468991 |
| Volume Number | 53 |
| DOI | 10.1007/s12088-012-0341-4 |
| PubMed Central reference number | PMC3587514 |
| Issue Number | 1 |
| PubMed reference number | 24426076 |
| Journal | Indian Journal of Microbiology [Indian J Microbiol] |
| e-ISSN | 09737715 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-12-15 |
| Publisher Place | India |
| Access Restriction | Open |
| Rights License | © Association of Microbiologists of India 2012 |
| Subject Keyword | Helicobacter pylori T4SS CagA CagL |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
