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Alteration in genes expression patterns during in vitro differentiation of mouse spermatogonial cells into neuroepithelial-like cells.
| Content Provider | Europe PMC |
|---|---|
| Author | Nazm Bojnordi, Maryam Movahedin, Mansoureh Tiraihi, Taki Javan, Mohamad |
| Abstract | Pluripotent stem cells derived from testis is a new, natural, and unlimited source for cell therapy in regenerative medicine and represent a possible alternative to replacing of all cells in the body. Here, we designed a simple co-culture system of spermatogonia cells with Sertoli cells for the generation of embryonic stem-like cells from mouse testis. The importance of our simple method will be clear when we compared it with other complex and time-consuming methods. Embryonic stem-like colonies with sharp border confirmed by real-time PCR, immunocytochemistry and flow cytometry assessments. Embryonic stem-like colonies were immunopositive for pluripotency markers. Transition of spermatogonia cells to embryonic stem-like cells was accompanied by extensive changes in gene expression. These changes included significant increase in pluripotency genes expression and significant decrease in germ cell-specific genes expression. Also, we proved the differentiation capacity of embryonic stem-like cells to neuroepithelial-like cells which were immunoreactive to Nestin and Neurofilament 68. Evaluation of genes expression during in vitro differentiation into neuroepithelial-like cells showed high-level expression of Nestin whether this gene approximately has no expression in undifferentiated embryonic stem-like cells. Also, expression of pluripotency genes has significantly decreased in neuroepithelial-like cells compared with embryonic stem-like cells. This study shows that embryonic stem-like cells derived from testis are capable to differentiate into neuroepithelial-like cells that may provide a cellular reservoir usable for neurodegenerative disorders. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3536871&blobtype=pdf |
| ISSN | 09209069 |
| Volume Number | 65 |
| DOI | 10.1007/s10616-012-9465-y |
| PubMed Central reference number | PMC3536871 |
| Issue Number | 1 |
| PubMed reference number | 23104269 |
| Journal | Cytotechnology |
| e-ISSN | 15730778 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2012-10-27 |
| Publisher Place | Dordrecht |
| Access Restriction | Open |
| Rights License | © Springer Science+Business Media B.V. 2012 |
| Subject Keyword | Spermatogonia cells Embryonic stem-like cell Reprogramming Pluripotency Neuroepithelial cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology |
