Isolation and Characterization of Antibacterial Compound from a Mangrove-Endophytic Fungus, Penicillium chrysogenum MTCC 5108.

Content Provider	Europe PMC
Author	Devi, Prabha Rodrigues, Cheryl Naik, C. G. D’Souza, L.
Abstract	Microorganisms, especially endophytic fungi that reside in the tissue of living mangrove plants, seem to play a major role in meeting the general demand for new biologically active substances. During the course of screening for biologically active secondary metabolites from marine microorganisms, an antibiotic compound containing an indole and a diketopiperazine moiety was isolated from the culture medium of Penicilliumchrysogenum, (MTCC 5108), an endophytic fungus on the mangrove plant Porteresiacoarctata (Roxb.). The cell free culture medium of P. chrysogenum showed significant activity against Vibriocholerae, (MCM B-322), a pathogen causing cholera in humans. Bioassay guided chemical characterization of the crude extract led to the isolation of a secondary metabolite possessing a molecular formula C19H21O2N3. Its antibacterial activity was comparable with standard antibiotic, streptomycin. This compound (1) was found to be (3,1′-didehydro-3[2″(3′″,3′″-dimethyl-prop-2-enyl)-3″-indolylmethylene]-6-methyl pipera-zine-2,5-dione) on the basis of mass spectrometry, infrared spectroscopy and one and two-dimensional nuclear magnetic resonance analysis.
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ISSN	00468991
Volume Number	52
DOI	10.1007/s12088-012-0277-8
PubMed Central reference number	PMC3516668
Issue Number	4
PubMed reference number	24293720
Journal	Indian Journal of Microbiology [Indian J Microbiol]
e-ISSN	09737715
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-06-21
Publisher Place	India
Access Restriction	Open
Rights License	© Association of Microbiologists of India 2012
Subject Keyword	Marine fungi Penicillium chrysogenum Secondary metabolite Vibrio cholerae
Content Type	Text
Resource Type	Article
Subject	Microbiology