NDLI: Evaluation of immunoprotective activity of six leptospiral proteins in the hamster model of leptospirosis.

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Evaluation of immunoprotective activity of six leptospiral proteins in the hamster model of leptospirosis.

Content Provider	Europe PMC
Author	Atzingen, Marina V Vieira, Mônica L Oliveira, Rosane Domingos, Renan F Mendes, Renata S Barros, Aline T Gonçales, Amane P de Morais, Zenaide M Vasconcellos, Silvio A Nascimento, Ana LTO
Copyright Year	2012
Abstract	Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira. The whole-genome sequence of L. interrogans serovar Copenhageni together with bioinformatics tools represent a great opportunity to search for novel antigen candidates that could be used as subunit vaccine against leptospirosis. We focused on six genes encoding for conserved hypothetical proteins predicted to be exported to the outer membrane. The genes were amplified by PCR from Leptospira interrogans genomic DNA and were cloned and expressed in Escherichia coli. The recombinant proteins tagged with N-terminal hexahistidine were purified by metal-charged chromatography. The immunization of hamsters followed by challenge with lethal dose of virulent strain of Leptospira showed that the recombinant proteins Lsa21, Lsa66 and rLIC11030 elicited partial protection to animals. These proteins could be used combined or in a mixture with novel adjuvants in order to improve their effectiveness.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3502890&blobtype=pdf
Journal	The Open Microbiology Journal [Open Microbiol J]
Volume Number	6
DOI	10.2174/1874285801206010079
PubMed Central reference number	PMC3502890
PubMed reference number	23173023
e-ISSN	18742858
Language	English
Publisher	Bentham Open
Publisher Date	2012-10-19
Access Restriction	Open
Rights License	This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited. © Atzingen et al.; Licensee Bentham Open.
Subject Keyword	Leptospira interrogans leptospirosis recombinant protein vaccine.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology

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