Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury.

Content Provider	Europe PMC
Author	Qin, Jin-Bao Li, Kang-An Li, Xiang-Xiang Xie, Qing-Song Lin, Jia-Ying Ye, Kai-Chuang Jiang, Mi-Er Zhang, Gui-Xiang Lu, Xin-Wu
Copyright Year	2012
Abstract	BackgroundStem cell therapy has shown great promise for regenerative repair of injured or diseased tissues. Adipose-derived stem cells (ADSCs) have become increasingly attractive candidates for cellular therapy. Magnetic resonance imaging has been proven to be effective in tracking magnetic-labeled cells and evaluating their clinical relevance after cell transplantation. This study investigated the feasibility of imaging green fluorescent protein-expressing ADSCs (GFP-ADSCs) labeled with superparamagnetic iron oxide particles, and tracked them in vivo with noninvasive magnetic resonance imaging after cell transplantation in a model of mouse carotid artery injury.MethodsGFP-ADSCs were isolated from the adipose tissues of GFP mice and labeled with superparamagnetic iron oxide particles. Intracellular stability, proliferation, and viability of the labeled cells were evaluated in vitro. Next, the cells were transplanted into a mouse carotid artery injury model. Clinical 3 T magnetic resonance imaging was performed immediately before and 1, 3, 7, 14, 21, and 30 days after cell transplantation. Prussian blue staining and histological analysis were performed 7 and 30 days after transplantation.ResultsGFP-ADSCs were found to be efficiently labeled with superparamagnetic iron oxide particles, with no effect on viability and proliferation. Homing of the labeled cells into the injured carotid artery tissue could be monitored by magnetic resonance imaging.ConclusionMagnetically labeled ADSCs with expression of GFP can home into sites of vascular injury, and may provide new insights into understanding of cell-based therapy for cardiovascular lesions.
ISSN	11769114
Journal	International Journal of Nanomedicine
Volume Number	7
PubMed Central reference number	PMC3487538
PubMed reference number	23125528
e-ISSN	11782013
DOI	10.2147/ijn.s35647
Language	English
Publisher	Dove Medical Press
Publisher Date	2012-10-02
Access Restriction	Open
Rights License	This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. © 2012 Qin et al, publisher and licensee Dove Medical Press Ltd.
Subject Keyword	adipose-derived stem cells carotid artery injury magnetic resonance imaging iron oxide particles cell therapy
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Organic Chemistry Drug Discovery Medicine Biomaterials Biophysics Bioengineering Pharmaceutical Science