Identification and Discrimination of Methicillin Resistant Staphylococcus aureus Strains Isolated from Burn Wound Sites Using PCR and Authentication with MALDI-TOF-MS.

Content Provider	Europe PMC
Author	Madhava Charyulu, E. Gnanamani, Arumugam Mandal, A. B.
Abstract	The present study demonstrates isolation and identification of methicillin resistance Staphylococcus aureus (MRSA) strains in the samples collected from burn patients. About 106 swab samples were collected from burn patients of >40% burn injury and were subjected to isolation using nutrient agar followed by screening using Me Re Sa selective medium agar. A total of 10 isolates with identity to S. aureus were obtained and further authenticated using Polymerase Chain Reaction and matrix assisted laser desorption/ionization time of flight mass spectrometry analysis. Presence of mec A gene and the peak pattern observations suggested seven of the 10 isolates are MRSA. Thus, the present study emphasizes the process of identification of MRSA using two different bio-analytical techniques, which authenticate the presence of MRSA.
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ISSN	00468991
Volume Number	52
DOI	10.1007/s12088-011-0245-8
PubMed Central reference number	PMC3460133
Issue Number	3
PubMed reference number	23997322
Journal	Indian Journal of Microbiology [Indian J Microbiol]
e-ISSN	09737715
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-01-14
Publisher Place	India
Access Restriction	Open
Rights License	© Association of Microbiologists of India 2012
Subject Keyword	Burn injury Me Re Sa selective medium S. aureus PCR MALDI-TOF–MS Methicillin resistance Staphylococcusaureus
Content Type	Text
Resource Type	Article
Subject	Microbiology