NDLI: Caprine cardiac sarcoplasmic reticulum isolation and biochemical characterisation with emphasis on Ca(2+)-adenosine triphosphatase.

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Caprine cardiac sarcoplasmic reticulum isolation and biochemical characterisation with emphasis on Ca(2+)-adenosine triphosphatase.

Content Provider	Europe PMC
Author	D'Souza, Karen M. Ashavaid, Tester F.
Abstract	This study was aimed at isolating, in its pure form, and characterizing the sarcoplasmic reticulum from caprine (Capra hircus) heart. The sarcoplasmic reticulum from thirty caprine heart ventricular homogenates was isolated and purified. It was characterized on the basis of both, its protein and lipid composition. The protein content was 142±10 mg/g of tissue. Ca2+-ATPase activity equaled 3.75±1.06mmol Pi/mg protein/min while the uptake rate was 24±1.14 nmol/mg protein/min. 205kD, 110kD, 90kD, 84kD, 66kD, 55kD and 29kD molecular weight proteins were seen on an SDS polyacrylamide gel. Triglyceride, Cholesterol and Phospholipids (phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, sphingomyelin and phosphatidylserine) were present in increasing order of their concentration. Long chain fatty acids predominated over the unsaturated ones. The ryanodine receptor displayed two binding sites for ryanodine. Characterisation encompassing the above biochemical aspects of normal caprine cardiac sarcoplasmic reticulum was thus achieved after isolating it in the pure form.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3454257&blobtype=pdf
ISSN	09701915
Volume Number	22
PubMed Central reference number	PMC3454257
Issue Number	1
PubMed reference number	23105650
Journal	Indian Journal of Clinical Biochemistry [Indian J Clin Biochem]
e-ISSN	09740422
DOI	10.1007/bf02912879
Language	English
Publisher	Springer India
Publisher Date	2007-03-01
Publisher Place	New Delhi
Access Restriction	Open
Rights License	© Association of Clinical BIochemists of India 2007
Subject Keyword	Sarcoplasmic reticulum Ca2+-ATPase Calcium Calcium homeostasis Excitation-contraction coupling
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry

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Caprine cardiac sarcoplasmic reticulum isolation and biochemical characterisation with emphasis on Ca$^{2+}$-adenosine triphosphatase

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