Detection of Helicobacter pylori using nested polymerase chain reaction in gastric biopsy samples.

Content Provider	Europe PMC
Author	Mahajan, Divya Jain, Anju Singh, Varsha Jain, A. K. Rao, G. R. K. Nath, Gopal
Abstract	Helicobacter pylori remains a controversial organism with regards to humans, its epidemiology still unclear nearly two decades after discovery. The present study was undertaken to estimate the prevalence of the organism in the gastrointestinal tract in symptomatic and asymptomatic subjects to understand its precise natural history in India. A total of 154 specimens were a part of the study. These included gastric biopsies from peptic ulcer disease and Non ulcer dyspepsia subjects, as visualized on endoscopy, saliva and stool samples from apparently normal healthy adults. Nested polymerase chain reaction was performed using the primers Hp1, Hp2, Hp3 targeting 16S rRNA gene. A prevalence of 65.1%, 100%, 66.7%, and 73.3% respectively was observed by polymerase chain reaction. No association was observed between the H.pylori status and the disease condition of the patient.
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ISSN	09701915
Volume Number	23
DOI	10.1007/s12291-008-0054-y
PubMed Central reference number	PMC3453439
Issue Number	3
PubMed reference number	23105762
Journal	Indian Journal of Clinical Biochemistry [Indian J Clin Biochem]
e-ISSN	09740422
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-07-01
Publisher Place	India
Access Restriction	Open
Rights License	© Association of Clinical Biochemists of India 2008
Subject Keyword	Helicobacter pylori Polymerase chain reaction Peptic ulcer disease Non ulcer dyspepsia
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry