Protective effect of ascorbic acid against oxidative damage induced by hydrogen peroxide in cultured human peripheral blood lymphocytes.

Content Provider	Europe PMC
Author	Siddique, Yasir Hasan Beg, Tanveer Afzal, Mohammad
Abstract	Hydrogen peroxide is one of the reactive oxygen species for cellular injury. It is overproduced during oxidative stress and is known to damage proteins, nucleic acids and cell membranes. The present study was aimed to study the protective effect of ascorbic acid against the toxic doses of hydrogen peroxide using lipid peroxidation and cytokinesis blocked micronucleus assay. Hydrogen peroxide was studied at 50, 100 and 200μM and was found to increase a dose dependent increase in lipid peroxidation and micronuclei frequency. The treatment of 100 and 200μM of hydrogen peroxide separately along with 20, 40 and 80μM of ascorbic acid showed a dose dependent decrease in the lipid peroxidation and micronuclei frequency. The results suggest a protective effect of ascorbic acid against the hydrogen peroxide induced oxidative damage in cultured human peripheral blood lymphocytes.
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ISSN	09701915
Volume Number	24
DOI	10.1007/s12291-009-0055-5
PubMed Central reference number	PMC3453308
Issue Number	3
PubMed reference number	23105852
Journal	Indian Journal of Clinical Biochemistry [Indian J Clin Biochem]
e-ISSN	09740422
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-07-01
Publisher Place	India
Access Restriction	Open
Rights License	© Association of Clinical Biochemists of India 2009
Subject Keyword	Hydrogen peroxide Ascorbic acid Lipid peroxidation Micronucleus Reactive oxygen species
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry