NDLI: Development of monoclonal antibody against isoquinoline alkaloid coptisine and its application for the screening of medicinal plants.

Please wait, while we are loading the content...

Development of monoclonal antibody against isoquinoline alkaloid coptisine and its application for the screening of medicinal plants.

Content Provider	Europe PMC
Author	Kim, Jun-Sik Tanaka, Hiroyuki Yuan, Chun-Su Shoyama, Yukihiro
Abstract	In the development of immunoassay technique, the design of hapten containing a functional group suitable for protein conjugate is the key step for the preparation of antibodies against small molecules. Coptisine (MW 320), a bioactive constituent of Berberis and Coptis species, is small as an immunogen. In addition, coptisine has no reactive group in molecule for conjugating with a protein. To overcome this problem, 9-O-carboxymethyl-berberrubine was designed and conjugated with carrier protein. In order to confirm its immunogenicity, the ratio of hapten in the conjugate was determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). After immunization, hybridomas secreting antibodies against coptisine were produced by fusing splenocytes with mouse myeloma cell line, P3-X63-Ag8-653. Among hybridomas, the clone 2A1 secreting anti-coptisine monoclonal antibody (MAb) 2A1-9E-1 was obtained through the limited dilution method. The MAb-based enzyme-linked immunosorbent assay (ELISA) against coptisine was developed and characterized. The linear range of the assay in this ELISA method was extended from 1.56 to 25 μg ml−1 possessing the detection limit of 1.56 μg ml−1. The established ELISA using MAb 2A1-9E-1 was applied for the survey of isoquinoline alkaloids in various medicinal plants.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3449483&blobtype=pdf
ISSN	09209069
Volume Number	44
DOI	10.1007/s10616-004-1179-3
PubMed Central reference number	PMC3449483
Issue Number	3
PubMed reference number	19003234
Journal	Cytotechnology
e-ISSN	15730778
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-03-01
Publisher Place	Dordrecht
Access Restriction	Open
Rights License	© Kluwer Academic Publishers 2004
Subject Keyword	Coptisine ELISA Isoquinoline alkaloid Monoclonal antibody Small hapten
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in