Histomorphometric analysis of the spine and femur in ovariectomized rats using micro-computed tomographic scan.

Content Provider	Europe PMC
Author	Shin, Yong-Hwan Cho, Dae-Chul Yu, Song-Hee Kim, Kyoung-Tae Cho, Hee-Jung Sung, Joo-Kyung
Copyright Year	2012
Abstract	ObjectiveThe purpose of this study was to evaluate the different patterns of bone loss between the lumbar spine and the femur after ovariectomy in rats.MethodsTwenty-four female Sprague-Dawley rats underwent a sham operation (the sham group) or bilateral ovariectomy (the ovariectomized group). Four and eight weeks after operation, six rats from each of the two groups were euthanized. Serum biochemical markers of bone turnover including osteocalcin and alkaline phosphatase (ALP), which are sensitive biochemical markers of bone formation, and the telopeptide fragment of type I collagen C-terminus (CTX), which is a sensitive biochemical marker of bone resorption, were analyzed. Bone histomorphometric parameters of the 4th lumbar vertebrae and femur were determined by micro-computed tomography.ResultsOvariectomized rats were found to have higher osteocalcin, ALP and CTX levels than sham controls. Additionally, 8 weeks after ovariectomy in the OVX group, serum levels of osteocalcin, ALP and CTX were significantly higher than those of 4 weeks after ovariectomy. Bone loss after ovariectomy was more extensive in the 4th lumbar spine compared to the femur. Bone loss in the 4th lumbar spine was mainly caused by trabecular thinning, but in the femur, it was mainly caused by trabecular elimination.ConclusionThe present study demonstrates different patterns of bone loss between the 4th lumbar spine and the femur in ovariectomized rats. Therefore, when considering animal models of osteoporosis, it is important that bone sites should be taken into account.
ISSN	20053711
Journal	Journal of Korean Neurosurgical Society
Volume Number	52
PubMed Central reference number	PMC3440496
Issue Number	1
PubMed reference number	22993670
e-ISSN	15987876
DOI	10.3340/jkns.2012.52.1.1
Language	English
Publisher	The Korean Neurosurgical Society
Publisher Date	2012-07-31
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2012 The Korean Neurosurgical Society
Subject Keyword	Bone loss Micro-CT Osteoporosis Ovariectomy Rat
Content Type	Text
Resource Type	Article
Subject	Neuroscience Neurology (clinical) Surgery