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Modified ionic liquid cold-induced aggregation dispersive liquid-liquid microextraction combined with spectrofluorimetry for trace determination of ofloxacin in pharmaceutical and biological samples.
| Content Provider | Europe PMC |
|---|---|
| Author | Zeeb, M. Ganjali, M.R. Norouzi, P. |
| Copyright Year | 2011 |
| Abstract | Background and the purpose of the studyOfloxacin is a quinolone synthetic antibiotic, which acts against resistant mutants of bacteria by inhibiting DNA gyrase. This antibacterial agent is widely used in the treatment of respiratory tract, urinary tract and tissue-based infections, which are caused by Gram-positive and Gram-negative bacteria. In this work, an efficient modified ionic liquid cold-induced aggregation dispersive liquid-liquid microextraction (M-IL-CIA-DLLME) was combined with spectrofluorimetry for trace determination of ofloxacin in real samples.MethodsIn this microextraction method, hydrophobic 1-hexyl-3-methylimidazolium hexafluorophosphate ([Hmim] [PF6]) ionic liquid (IL) as a microextraction solvent was dispersed into a heated sample solution containing sodium hexafluorophosphate (NaPF6) (as a common ion) and the analyte of interest. Afterwards, the resultant solution was cooled in an ice-water bath and a cloudy condition was formed due to a considerable decrease of IL solubility. After centrifuging, the enriched phase was introduced to the spectrofluorimeter for the determination of ofloxacin.Results and major conclusionIn this technique, the performance of the microextraction method was not influenced by variations in the ionic strength of the sample solution (up to 30% w/v). Furthermore, [Hmim][PF6] IL was chosen as a green microextraction phase and an alternative to traditional toxic organic solvents. Different parameters affecting the analytical performance were studied and optimized. At optimum conditions, a relatively broad linear dynamic range of 0.15-125 µg l-1 and a limit of detection (LOD) of 0.029 µg l-1 were obtained. The relative standard deviation (R.S.D.) obtained for the determination of five replicates of the 10 ml solution containing 50 µg l-1 ofloxacin was 2.7%. Finally, the combined methodology was successfully applied to ofloxacin determination in actual pharmaceutical formulations and biological samples. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3436082&blobtype=pdf |
| ISSN | 15608115 |
| Journal | DARU Journal of Pharmaceutical Sciences [Daru] |
| Volume Number | 19 |
| PubMed Central reference number | PMC3436082 |
| Issue Number | 6 |
| PubMed reference number | 23008691 |
| e-ISSN | 20082231 |
| Language | English |
| Publisher | Tehran University of Medical Sciences |
| Publisher Date | 2011-01-01 |
| Access Restriction | Open |
| Rights License | This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. © 2011 Tehran University of Medical Sciences |
| Subject Keyword | 1-Hexyl-3-methylimidazolium hexafluorophosphate Common ion Real samples |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Drug Discovery Pharmacology |
