Recent advances in rice genome and chromosome structure research by fluorescence in situ hybridization (FISH).

Content Provider	Europe PMC
Author	Ohmido, Nobuko Fukui, Kiichi Kinoshita, Toshiro
Copyright Year	2010
Abstract	Fluorescence in situ hybridization (FISH) is an effective method for the physical mapping of genes and repetitive DNA sequences on chromosomes. Physical mapping of unique nucleotide sequences on specific rice chromosome regions was performed using a combination of chromosome identification and highly sensitive FISH. Increases in the detection sensitivity of smaller DNA sequences and improvements in spatial resolution have ushered in a new phase in FISH technology. Thus, it is now possible to perform in situ hybridization on somatic chromosomes, pachytene chromosomes, and even on extended DNA fibers (EDFs). Pachytene-FISH allows the integration of genetic linkage maps and quantitative chromosome maps. Visualization methods using FISH can reveal the spatial organization of the centromere, heterochromatin/euchromatin, and the terminal structures of rice chromosomes. Furthermore, EDF-FISH and the DNA combing technique can resolve a spatial distance of 1 kb between adjacent DNA sequences, and the detection of even a 300-bp target is now feasible. The copy numbers of various repetitive sequences and the sizes of various DNA molecules were quantitatively measured using the molecular combing technique. This review describes the significance of these advances in molecular cytology in rice and discusses future applications in plant studies using visualization techniques.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3417561&blobtype=pdf
ISSN	03862208
Journal	Proceedings of the Japan Academy. Series B, Physical and Biological Sciences [Proc Jpn Acad Ser B Phys Biol Sci]
Volume Number	86
DOI	10.2183/pjab.86.103
PubMed Central reference number	PMC3417561
Issue Number	2
PubMed reference number	20154468
e-ISSN	13492896
Language	English
Publisher	The Japan Academy
Publisher Date	2010-01-01
Publisher Place	Tokyo, Japan
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2010 The Japan Academy
Subject Keyword	Oryza sativa L. chromosome fluorescence in situ hybridization (FISH) extended DNA fibers (EDFs) genomic in situ hybridization (GISH)
Content Type	Text
Resource Type	Article
Subject	Physics and Astronomy Medicine Agricultural and Biological Sciences