RhoB regulates cell migration through altered focal adhesion dynamics.

Content Provider	Europe PMC
Author	Vega, Francisco M. Colomba, Audrey Reymond, Nicolas Thomas, Mairian Ridley, Anne J.
Copyright Year	2012
Abstract	The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension, although they have active membrane ruffling around the periphery. Depletion of the exchange factor GEF-H1 induces a similar phenotype. RhoB-depleted cells migrate faster, but less persistently in a chemotactic gradient, and frequently round up during migration. RhoB-depleted cells have similar numbers of focal adhesions to control cells during spreading and migration, but show more diffuse and patchy contact with the substratum. They have lower levels of surface β1 integrin, and β1 integrin activity is reduced in actin-rich protrusions. We propose that RhoB contributes to directional cell migration by regulating β1 integrin surface levels and activity, thereby stabilizing lamellipodial protrusions.
Journal	Open Biology
Volume Number	2
PubMed Central reference number	PMC3376739
Issue Number	5
PubMed reference number	22724071
e-ISSN	20462441
DOI	10.1098/rsob.120076
Language	English
Publisher	The Royal Society
Publisher Date	2012-05-01
Access Restriction	Open
Rights License	© 2012 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.
Subject Keyword	Rho guanosine triphosphate cytoskeleton RhoB focal adhesions integrins Rac1
Content Type	Text
Resource Type	Article
Subject	Immunology Biochemistry, Genetics and Molecular Biology Neuroscience