NDLI: Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles.

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Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles.

Content Provider	Europe PMC
Author	Huang, Kai-Wen Yang, Shieh-Yueh Hong, Yu-Wei Chieh, Jen-Jie Yang, Che-Chuan Horng, Herng-Er Wu, Chau-Chung Hong, Chin-Yih Yang, Hong-Chang
Copyright Year	2012
Abstract	Some previous reports have already shown the characterizations of immunomagnetic reduction (IMR). The assay technology involves the utilities of biofunctionalized magnetic nanoparticles to label target biomolecules. However, the detection threshold and interference tests for IMR have not been investigated in detail. In this study, alpha-fetoprotein (AFP) was used as a target biomolecule. The signals for AFP solutions of various concentrations, or with interfering materials, were detected via IMR. These samples were also used for characterizing the detection threshold and interference with enzyme-linked immunosorbent assay (ELISA). The results of assaying AFP level with IMR and ELISA were compared. The detection threshold for assaying AFP with IMR was found to be 3 ng/mL, which is 15 times lower than that of ELISA, and definitely suppresses false negative. For the interfering materials noted commonly in serum such as hemoglobin, bilirubin, triglyceride, and vascular endothelial growth factor, there was no detectable interfering effect when assaying AFP with IMR. Several serum samples from normal people and liver-tumor-bearing patients were used for the detections of AFP concentration via IMR. These results reveal the feasibilities of assaying AFP in blood using IMR, as well as achieving high-sensitive and high-specific assay for AFP.
ISSN	11769114
Journal	International Journal of Nanomedicine
Volume Number	7
PubMed Central reference number	PMC3356210
PubMed reference number	22619536
e-ISSN	11782013
DOI	10.2147/ijn.s28245
Language	English
Publisher	Dove Medical Press
Publisher Date	2012-04-17
Access Restriction	Open
Rights License	This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. © 2012 Huang et al, publisher and licensee Dove Medical Press Ltd.
Subject Keyword	immunomagnetic reduction ELISA biofunctionalized magnetic nanoparticles
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Organic Chemistry Drug Discovery Medicine Biomaterials Biophysics Bioengineering Pharmaceutical Science

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