Imbalanced expression of mitogen-activated protein kinase phosphatase-1 and phosphorylated extracellular signal-regulated kinases in lung squamous cell carcinoma.

Content Provider	Europe PMC
Author	Wang, Kai Zhang, Min Qian, Ying-ying Ding, Zhe-yuan Lv, Jun-hui Shen, Hua-hao
Copyright Year	2011
Abstract	Objective: Mitogen-activated protein kinases (MAPKs) are correlated with a more malignant phenotype in many cancers. This study was designed to evaluate the predictive value of the expression of MAPK phosphatase-1 (MKP-1) and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), as the key regulatory mechanism of the MAPKs, in lung squamous cell carcinoma (SCC). Methods: We assessed the expressions of MKP-1 and p-ERK1/2 in twenty subjects at different differentiation degree of SCC and five normal lungs by immunohistochemistry and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis. Results: Immunohistochemistry and real-time RT-PCR assay showed that the expression of MKP-1 was gradually decreased as tissue type went from normal lung tissues to increasingly undifferentiated carcinoma, and it was negatively correlated with tumor differentiation (P<0.01). However, the expression of p-ERK1/2 or ERK1/2 was gradually increased as tissue type went from normal lung tissues to increasingly undifferentiated carcinoma, and it was positively correlated with tumor differentiation (P<0.01). Conclusions: Our data indicates the relevance of MKP-1 and p-ERK1/2 in SCC as a potential positive and negative prognostic factor. The imbalanced expression of MKP-1 and p-ERK1/2 may play a role in the development of SCC and these two molecules may be new targets for the therapy and prognosis of SCC.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3190098&blobtype=pdf
ISSN	16731581
Volume Number	12
PubMed Central reference number	PMC3190098
Issue Number	10
PubMed reference number	21960346
Journal	Journal of Zhejiang University. Science. B [J Zhejiang Univ Sci B]
e-ISSN	18621783
DOI	10.1631/jzus.b1100165
Language	English
Publisher	Zhejiang University Press
Publisher Date	2011-10-01
Publisher Place	Hangzhou
Access Restriction	Open
Rights License	Copyright © Zhejiang University and Springer-Verlag Berlin Heidelberg 2011
Subject Keyword	Mitogen-activated protein kinase phosphatase-1 (MKP-1) Extracellular signal-regulated kinase (ERK) Lung squamous cell carcinoma (SCC) Prognostic factor
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics