NDLI: CARS microscopy for the visualization of micrometer-sized iron oxide MRI contrast agents in living cells.

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CARS microscopy for the visualization of micrometer-sized iron oxide MRI contrast agents in living cells.

Content Provider	Europe PMC
Author	Rago, Gianluca Langer, Carolin M. Brackman, Christian Day, James P.R. Domke, Katrin F. Raschzok, Nathanael Schmidt, Christian Sauer, Igor M. Enejder, Annika Mogl, Martina T. Bonn, Mischa
Copyright Year	2011
Abstract	Micrometer-sized iron oxide particles (MPIOs) attract increasing interest as contrast agents for cellular tracking by clinical Magnetic Resonance Imaging (MRI). Despite the great potential of MPIOs for in vivo imaging of labeled cells, little is known on the intracellular localization of these particles following uptake due to the lack of techniques with the ability to monitor the particle uptake in vivo at single-cell level. Here, we show that coherent anti-Stokes Raman scattering (CARS) microscopy enables non-invasive, label-free imaging of MPIOs in living cells with sub-micron resolution in three dimensions. CARS allows simultaneous visualization of the cell framework and the MPIOs, where the particles can be readily distinguished from other cellular components of comparable dimensions, and localized inside the cell.
Journal	Biomedical Optics Express
Volume Number	2
PubMed Central reference number	PMC3184857
Issue Number	9
PubMed reference number	21991541
e-ISSN	21567085
DOI	10.1364/BOE.2.002470
Language	English
Publisher	Optical Society of America
Publisher Date	2011-07-29
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. ©2011 Optical Society of America
Subject Keyword	(300.6230) Spectroscopy, coherent anti-Stokes Raman scattering (180.6900) Three-dimensional microscopy (020.4180) Multiphoton processes (170.3880) Medical and biological imaging (190.4380) Nonlinear optics, four-wave mixing (160.4236) Nanomaterials
Content Type	Text
Resource Type	Article
Subject	Biotechnology Atomic and Molecular Physics, and Optics

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