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Characterization of the mouse gene for the heavy metal-responsive transcription factor MTF-1.
| Content Provider | Europe PMC |
|---|---|
| Author | der Maur, Adrian Auf Belser, Tanja Wang, Ying Günes, Cagatay Lichtlen, Peter Georgiev, Oleg Schaffner, Walter |
| Abstract | MTF-1 is a zinc finger transcription factor that mediates the cellular response to heavy metal stress; its targeted disruption in the mouse leads to liver decay and embryonic lethality at day E14. Recently, we have sequenced the entire MTF-1 gene in the compact genome of the pufferfish Fugu rubripes. Here we have defined the promoter sequences of human and mouse MTF-1 and the genomic structure of the mouse MTF-1 locus. The transcription unit of MTF-1 spans 42 kb (compared to 8.5 kb in Fugu) and is located downstream of the gene for a phosphatase (INPP5P) in mouse, human, and fish. In all of these species, the MTF promoter region has the features of a CpG island. In both mouse and human, the 5′ untranslated region harbors conserved short reading frames of unknown function. RNA mapping experiments revealed that in these two species, MTF-1 mRNA is transcribed from a cluster of multiple initiation sites from a TATA-less promoter without metal-responsive elements. Transcription from endogenous and transfected MTF-1 promoters was not affected by heavy metal load or other stressors, in support of the notion that MTF-1 activity is regulated at the posttranscriptional level. Tissue Northern blots normalized for poly A+ RNA indicate that MTF-1 is expressed at similar levels in all tissues, except in the testes, that contain more than 10-fold higher mRNA levels. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC312886&blobtype=pdf |
| ISSN | 13558145 |
| Volume Number | 5 |
| PubMed Central reference number | PMC312886 |
| Issue Number | 3 |
| PubMed reference number | 11005378 |
| Journal | Cell Stress Chaperones |
| e-ISSN | 14661268 |
| DOI | 10.1379/1466-1268(2000)005<0196:cotmgf>2.0.co;2 |
| Language | English |
| Publisher | Cell Stress Society International |
| Publisher Date | 2000-07-01 |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry |
