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In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes.
| Content Provider | Europe PMC |
|---|---|
| Author | Khatun, Momena Bhuiyan, Mohammad Musharraf Uddin Ahmed, Jalal Uddin Haque, Aminul Rahman, Mohammad Bozlur Shamsuddin, Mohammed |
| Copyright Year | 2011 |
| Abstract | Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle's salt with L-glutamine and sodium bicarbonate) for 27 h at 39℃ under 5% CO2 in humidified air. The oocytes retrieval and efficiency (mean ± SD) per prepubertal and pubertal goats were 5.2 ± 0.6 and 6.8 ± 0.6, and 77.3 ± 0.1% and 80.5 ± 0.6%, respectively. Anaphase I - telophase I stages differed significantly (7.3 ± 0.8 vs. 2.6 ± 0.2, p < 0.05) between the two groups of goats. After IVM, the percentages of metaphase II were significantly higher (66.3 vs. 60.3, p < 0.05) in pubertal goats than in their prepubertal counterparts. The percentages of normal in vitro fertilization (IVF) in Fert-Tyrode's albumin lactate pyruvate of pubertal goat oocytes did not differ between Percoll and swim-up sperm separation methods (36.7 ± 0.9% vs. 32.7 ± 1.3%, p > 0.05). Furthermore, sperm capacitation by heparin alone or in combination with ionomycin did not lead to a significant increase in the normal fertilization rate (34.8 ± 1.7 vs. 32.2 ± 1.5%, respectively) in the oocytes of pubertal goats. In conclusion, the ovaries of pubertal black Bengal goats obtained from the slaughterhouse could be used for in vitro embryo production. However, further optimization of the IVM and IVF techniques are necessary for satisfactory in vitro embryo production. |
| ISSN | 1229845X |
| Journal | Journal of Veterinary Science |
| Volume Number | 12 |
| PubMed Central reference number | PMC3053471 |
| Issue Number | 1 |
| PubMed reference number | 21368566 |
| e-ISSN | 1976555X |
| DOI | 10.4142/jvs.2011.12.1.75 |
| Language | English |
| Publisher | The Korean Society of Veterinary Science |
| Publisher Date | 2011-03-01 |
| Access Restriction | Open |
| Rights License | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2011 The Korean Society of Veterinary Science |
| Subject Keyword | black Bengal goat IVF IVM nuclear status oocyte retrieval |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary |
