NDLI: Crystallization and preliminary X-ray crystallographic studies of enoyl-acyl carrier protein reductase (FabI) from Psuedomonas aeruginosa.

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Crystallization and preliminary X-ray crystallographic studies of enoyl-acyl carrier protein reductase (FabI) from Psuedomonas aeruginosa.

Content Provider	Europe PMC
Author	Lee, Jeong Hye Park, Ae Kyung Chi, Young Min Moon, Jin Ho Lee, Ki Seog
Copyright Year	2011
Description	Enoyl-acyl carrier protein reductase (FabI) from P. aeruginosa was purified and crystallized. FabI was also cocrystallized with the inhibitor triclosan and the cofactor NADH. Crystals of native and complexed FabI diffracted to resolutions of 2.6 and 1.8 Å, respectively. During fatty-acid biosynthesis, enoyl-acyl carrier protein (enoyl-ACP) reductase catalyzes the reduction of trans-2-enoyl-ACP to fully saturated acyl-ACP via the ubiquitous fatty-acid synthase system. NADH-dependent enoyl-ACP reductase (FabI) from Pseudomonas aeruginosa has been purified and crystallized as an apoenzyme and in a complex form with NADH and triclosan. Triclosan is an inhibitor of FabI and forms a stable ternary complex in the presence of NADH. The crystals of native and complexed FabI diffracted to resolutions of 2.6 and 1.8 Å, respectively. The crystals both belonged to space group P21, with unit-cell parameters a = 117.32, b = 155.844, c = 129.448 Å, β = 111.061° for the native enzyme and a = 64.784, b = 107.573, c = 73.517 Å, β = 116.162° for the complex. Preliminary molecular replacement further confirmed the presence of four tetramers of native FabI and one tetramer of the complex in the asymmetric unit, corresponding to Matthews coefficients (V M) of 2.46 and 2.05 Å3 Da−1 and solvent contents of 50.1 and 40.1%, respectively.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3034610&blobtype=pdf
Volume Number	67
PubMed Central reference number	PMC3034610
Issue Number	Pt 2
Issue Number	pt 2
PubMed reference number	21301088
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun]
e-ISSN	17443091
DOI	10.1107/s1744309110048827
Language	English
Publisher	International Union of Crystallography
Publisher Date	2011-01-22
Access Restriction	Open
Rights License	© International Union of Crystallography 2011
Subject Keyword	enoyl-acyl carrier protein reductases FabI Pseudomonas aeruginosa fatty-acid synthesis triclosan
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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