NDLI: The structural origin of second harmonic generation in fascia.

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The structural origin of second harmonic generation in fascia.

Content Provider	Europe PMC
Author	Rivard, Maxime Laliberté, Mathieu Bertrand-Grenier, Antony Harnagea, Catalin Pfeffer, Christian P. Vallières, Martin St-Pierre, Yves Pignolet, Alain El Khakani, My Ali Légaré, François
Copyright Year	2010
Abstract	Fascia tissue is rich in collagen type I proteins and can be imaged by second harmonic generation (SHG) microscopy. While identifying the overall alignment of the collagen fibrils is evident from those images, the tridimensional structural origin for the observation of SHG signal is more complex than it apparently seems. Those images reveal that the noncentrosymmetric (piezoelectric) structures are distributed heterogeneously on spatial dimensions inferior to the resolution provided by the nonlinear optical microscope (sub-micron). Using piezoresponse force microscopy (PFM), we show that an individual collagen fibril has a noncentrosymmetric structural organization. Fibrils are found to be arranged in nano-domains where the anisotropic axis is preserved along the fibrillar axis, while across the collagen sheets, the phase of the second order nonlinear susceptibility is changing by 180 degrees between adjacent nano-domains. This complex architecture of noncentrosymmetric nano-domains governs the coherent addition of 2ω light within the focal volume and the observed features in the SHG images taken in fascia.
Journal	Biomedical Optics Express
Volume Number	2
PubMed Central reference number	PMC3028495
Issue Number	1
PubMed reference number	21326632
e-ISSN	21567085
DOI	10.1364/BOE.2.000026
Language	English
Publisher	Optical Society of America
Publisher Date	2010-12-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. ©2010 Optical Society of America
Subject Keyword	(180.4315) Nonlinear microscopy (190.4160) Multiharmonic generation
Content Type	Text
Resource Type	Article
Subject	Biotechnology Atomic and Molecular Physics, and Optics

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