NDLI: Transcriptional activity of Plasmodium subtilisin-like protease 2 (Pf-Sub2) 5'untranslated regions and its interaction with hepatocyte growth factor.

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Transcriptional activity of Plasmodium subtilisin-like protease 2 (Pf-Sub2) 5'untranslated regions and its interaction with hepatocyte growth factor.

Content Provider	Europe PMC
Author	Liao, Shunyao Liu, Yunqiang Jung, Suk-Yul Cho, Pyo Yun Zheng, Bing Park, Hyun
Copyright Year	2010
Abstract	The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors and individual host responses to these determinants. In both humans and mice, liver injury is involved after parasite entry, which persists until the erythrocyte stage after infection with the fatal strain Plasmodium falciparum (Pf). Hepatocyte growth factor (HGF) has strong anti-apoptotic effects in various kinds of cells, and also has diverse metabolic functions. In this work, Pf-subtilisin-like protease 2 (Pf-Sub2) 5'untranslated region (UTR) was analyzed and its transcriptional activity was estimated by luciferase expression. Fourteen TATA boxes were observed but only one Oct-1 and c-Myb were done. In addition, host HGF interaction with Pf-Sub2 was evaluated by co-transfection of HGF- and Pf-Sub2-cloned vector. Interestingly, -1,422/+12 UTR exhibited the strongest luciferase activity but -329 to +12 UTR did not exhibit luciferase activity. Moreover, as compared with the control of unexpressed HGF, the HGF protein suppressed luciferase expression driven by the 5'untranslated region of the Pf-Sub2 promoter. Taken together, it is suggested that HGF controls and interacts with the promoter region of the Pf-Sub2 gene.
ISSN	00234001
Journal	The Korean Journal of Parasitology
Volume Number	48
PubMed Central reference number	PMC3018577
Issue Number	4
PubMed reference number	21234230
e-ISSN	17380006
DOI	10.3347/kjp.2010.48.4.291
Language	English
Publisher	The Korean Society for Parasitology
Publisher Date	2010-12-16
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2010, Korean Society for Parasitology
Subject Keyword	Plasmodium falciparum malaria subtilisin-like protease 2 hepatocyte growth factor 5'untranslated reagion promoter
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology

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