High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process.

Content Provider	Europe PMC
Author	Isobe, Keisuke Suda, Akira Hashimoto, Hiroshi Kannari, Fumihiko Kawano, Hiroyuki Mizuno, Hideaki Miyawaki, Atsushi Midorikawa, Katsumi
Copyright Year	2010
Abstract	We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton (CSM) process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore induced by pump and reverse light. By temporally modulating the reverse intensity, we can extract the fluorescence signal generated through the CSM process. We show that the demodulated fluorescence signal is nonlinearly proportional to the excitation intensities and it gives a higher spatial resolution than that of a confocal microscope.
Journal	Biomedical Optics Express
Volume Number	1
PubMed Central reference number	PMC3018047
Issue Number	3
PubMed reference number	21258510
e-ISSN	21567085
DOI	10.1364/BOE.1.000791
Language	English
Publisher	Optical Society of America
Publisher Date	2010-09-07
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. ©2010 Optical Society of America
Subject Keyword	(180.2520) Fluorescence microscopy (180.6900) Three-dimensional microscopy (120.1880) Detection
Content Type	Text
Resource Type	Article
Subject	Biotechnology Atomic and Molecular Physics, and Optics