NDLI: Purification, crystallization and preliminary X-ray analysis of the dissimilatory sulfite reductase from Desulfovibrio vulgaris Miyazaki F.

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Purification, crystallization and preliminary X-ray analysis of the dissimilatory sulfite reductase from Desulfovibrio vulgaris Miyazaki F.

Content Provider	Europe PMC
Author	Ogata, Hideaki Shomura, Yasuhito Goenka Agrawal, Aruna Kaur, Amrit Pal Gärtner, Wolfgang Higuchi, Yoshiki Lubitz, Wolfgang
Copyright Year	2010
Description	Dissimilatory sulfite reductase (Dsr) plays an important role in sulfate respiration in many sulfate-reducing bacteria. Dsr from Desulfovibrio vulgaris Miyazaki F has been purified and crystallized at 277 K using the sitting-drop vapour-diffusion method with PEG 3350 and potassium thiocyanate as precipitants. A data set was collected to 3.7 Å resolution from a single crystal at 100 K using synchrotron radiation. The Dsr crystal belonged to space group P41212, with unit-cell parameters a = b = 163.26, c = 435.32 Å. The crystal structure of Dsr was determined by the molecular-replacement method based on the three-dimensional structure of Dsr from D. vulgaris Hildenborough. The crystal contained three α2β2γ2 units per asymmetric unit, with a Matthews coefficient (V M) of 2.35 Å3 Da−1; the solvent content was estimated to be 47.7%. Dissimilatory sulfite reductase from Desulfovibrio vulgaris Miyazaki F was purified and crystallized. The crystals belonged to the space group P41212 and diffracted to 3.7 Å resolution.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3001650&blobtype=pdf
Volume Number	66
PubMed Central reference number	PMC3001650
Issue Number	Pt 11
Issue Number	pt 11
PubMed reference number	21045297
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun]
e-ISSN	17443091
DOI	10.1107/s1744309110033191
Language	English
Publisher	International Union of Crystallography
Publisher Date	2010-10-28
Access Restriction	Open
Rights License	© International Union of Crystallography 2010
Subject Keyword	dissimilatory sulfite reductases sulfite reduction sulfate-reducing bacteria
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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