NDLI: Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant.

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Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant.

Content Provider	Europe PMC
Author	Protsko, Carla Vieille, Claire Laivenieks, Maris Prasad, Lata Sanders, David A. R. Delbaere, Louis T. J.
Copyright Year	2010
Description	The secondary alcohol dehydrogenase mutant I86A from Thermoanaerobacter ethanolicus (TeSADH) was crystallized in novel crystallization conditions. Diffraction data to 3.2 Å were collected at the Canadian Light Source. The Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols. Pyramidal crystals grown in the presence of (R)-phenylethanol via the hanging-drop vapour-diffusion method diffracted to 3.2 Å resolution at the Canadian Light Source. The crystal belonged to the orthorhombic space group P212121, with unit-cell parameters a = 80.23, b = 124.90, c = 164.80 Å. The structure was solved by molecular replacement using the structure of T. brockii SADH (PDB entry 1ykf).
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2898473&blobtype=pdf
Volume Number	66
PubMed Central reference number	PMC2898473
Issue Number	Pt 7
Issue Number	pt 7
PubMed reference number	20606285
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun]
e-ISSN	17443091
DOI	10.1107/s1744309110018981
Language	English
Publisher	International Union of Crystallography
Publisher Date	2010-06-24
Access Restriction	Open
Rights License	© International Union of Crystallography 2010
Subject Keyword	secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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