Crystallization and preliminary crystallographic analysis of the Clostridium perfringens enterotoxin.

Content Provider	Europe PMC
Author	Briggs, David C. Smedley, James G. McClane, Bruce A. Basak, Ajit K.
Copyright Year	2010
Description	The crystallization of the C. perfringens enterotoxin is reported. Clostridium perfringens is a Gram-positive anaerobic species of bacterium that is notable for its ability to produce a plethora of toxins, including membrane-active toxins (α-toxins), pore-forming toxins (∊-toxins) and binary toxins (ι-toxins). Here, the crystallization of the full-length wild-type C. perfringens enterotoxin is reported, which is the causative agent of the second most prevalent food-borne illness in the United States and has been implicated in many other gastrointestinal pathologies. Several crystal forms were obtained. However, only two of these optimized crystal forms (I and II) were useable for X-ray diffraction data collection. The form I crystals diffracted to d min = 2.7 Å and belonged to space group C2, while the form II crystals diffracted to d min = 4 Å and belonged to space group P213.
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Volume Number	66
PubMed Central reference number	PMC2898463
Issue Number	Pt 7
Issue Number	pt 7
PubMed reference number	20606275
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun]
e-ISSN	17443091
DOI	10.1107/s1744309110016507
Language	English
Publisher	International Union of Crystallography
Publisher Date	2010-06-24
Access Restriction	Open
Rights License	© International Union of Crystallography 2010
Subject Keyword	pore-forming toxins enterotoxins Clostridium perfringens
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology