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The 1.4 A resolution structure of Paracoccus pantotrophus pseudoazurin.
| Content Provider | Europe PMC |
|---|---|
| Author | Najmudin, Shabir Pauleta, Sofia R. Moura, Isabel Romão, Maria J. |
| Copyright Year | 2010 |
| Description | A high-resolution structure of P. pantrotrophus pseudoazurin is reported at 1.4 Å resolution, together with secondary-structure-based sequence alignment of the type 1 copper protein family. Pseudoazurins are small type 1 copper proteins that are involved in the flow of electrons between various electron donors and acceptors in the bacterial periplasm, mostly under denitrifying conditions. The previously determined structure of Paracoccus pantotrophus pseudoazurin in the oxidized form was improved to a nominal resolution of 1.4 Å, with R and R free values of 0.188 and 0.206, respectively. This high-resolution structure makes it possible to analyze the interactions between the monomers and the solvent structure in detail. Analysis of the high-resolution structure revealed the structural regions that are responsible for monomer–monomer recognition during dimer formation and for protein–protein interaction and that are important for partner recognition. The pseudoazurin structure was compared with other structures of various type 1 copper proteins and these were grouped into families according to similarities in their secondary structure; this may be useful in the annotation of copper proteins in newly sequenced genomes and in the identification of novel copper proteins. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2882758&blobtype=pdf |
| Volume Number | 66 |
| PubMed Central reference number | PMC2882758 |
| Issue Number | Pt 6 |
| Issue Number | pt 6 |
| PubMed reference number | 20516588 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun] |
| e-ISSN | 17443091 |
| DOI | 10.1107/s1744309110013989 |
| Language | English |
| Publisher | International Union of Crystallography |
| Publisher Date | 2010-05-25 |
| Access Restriction | Open |
| Rights License | © International Union of Crystallography 2010 |
| Subject Keyword | pseudoazurin copper proteins Paracoccus pantotrophus electron transfer type 1 blue copper protein family |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology |
