NDLI: Crystallization and preliminary X-ray crystallographic study of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Plasmodium falciparum.

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Crystallization and preliminary X-ray crystallographic study of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Plasmodium falciparum.

Content Provider	Europe PMC
Author	Umeda, Tomonobu Tanaka, Nobutada Kusakabe, Yoshio Nakanishi, Masayuki Kitade, Yukio Nakamura, Kazuo T.
Copyright Year	2010
Description	1-Deoxy-d-xylulose 5-phosphate reductoisomerase from P. falciparum has been crystallized in the presence of NADPH. Diffraction data to 1.85 Å resolution have been collected using synchrotron radiation. The nonmevalonate pathway of isoprenoid biosynthesis present in Plasmodium falciparum is known to be an effective target for antimalarial drugs. The second enzyme of the nonmevalonate pathway, 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR), catalyzes the transformation of 1-deoxy-d-xylulose 5-phosphate (DXP) to 2-C-methyl-d-erythritol 4-phosphate (MEP). For crystallographic studies, DXR from the human malaria parasite P. falciparum (PfDXR) was overproduced in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method in the presence of NADPH. X-ray diffraction data to 1.85 Å resolution were collected from a monoclinic crystal form belonging to space group C2 with unit-cell parameters a = 168.89, b = 59.65, c = 86.58 Å, β = 117.8°. Structural analysis by molecular replacement is in progress.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2833050&blobtype=pdf
Volume Number	66
PubMed Central reference number	PMC2833050
Issue Number	Pt 3
Issue Number	pt 3
PubMed reference number	20208174
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun]
e-ISSN	17443091
DOI	10.1107/s1744309110001739
Language	English
Publisher	International Union of Crystallography
Publisher Date	2010-02-25
Access Restriction	Open
Rights License	© International Union of Crystallography 2010
Subject Keyword	1-deoxy-d-xylulose 5-phosphate reductoisomerase malaria nonmevalonate pathway
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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