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Hematopoietic differentiation of embryoid bodies derived from the human embryonic stem cell line SNUhES3 in co-culture with human bone marrow stromal cells.
| Content Provider | Europe PMC |
|---|---|
| Author | Kim, Seok Jin Kim, Byung Soo Ryu, Suck Won Yoo, Ji Hyun Oh, Jee Hyun Song, Chang Hee Kim, Sun Haeng Choi, Dong Seop Seo, Jae Hong Choi, Chul Won Shin, Sang Won Kim, Yeul Hong Kim, Jun Suk |
| Copyright Year | 2005 |
| Abstract | Human embryonic stem (ES) cells can be induced to differentiate into hematopoietic precursor cells via two methods: the formation of embryoid bodies (EBs) and co-culture with mouse bone marrow (BM) stromal cells. In this study, the above two methods have been combined by co-culture of human ES-cell-derived EBs with human BM stromal cells. The efficacy of this method was compared with that using EB formation alone. The undifferentiated human ES cell line SNUhES3 was allowed to form EBs for two days, then EBs were induced to differentiate in the presence of a different serum concentration (EB and EB/high FBS group), or co-cultured with human BM stromal cells (EB/BM co-culture group). Flow cytometry and hematopoietic colony-forming assays were used to assess hematopoietic differentiation in the three groups. While no significant increase of CD34+/CD45- or CD34+/CD38- cells was noted in the three groups on days 3 and 5, the percentage of CD34+/CD45- cells and CD34+/CD38- cells was significantly higher in the EB/BM co-culture group than in the EB and EB/high FBS groups on day 10. The number of colony-forming cells (CFCs) was increased in the EB/BM co-culture group on days 7 and 10, implying a possible role for human BM stromal cells in supporting hematopoietic differentiation from human ES cell-derived EBs. These results demonstrate that co-culture of human ES-cell-derived EBs with human BM stromal cells might lead to more efficient hematopoietic differentiation from human ES cells cultured alone. Further study is warranted to evaluate the underlying mechanism. |
| ISSN | 05135796 |
| Volume Number | 46 |
| PubMed Central reference number | PMC2810577 |
| Issue Number | 5 |
| PubMed reference number | 16259069 |
| Journal | Yonsei Medical Journal [Yonsei Med. J] |
| e-ISSN | 19762437 |
| DOI | 10.3349/ymj.2005.46.5.693 |
| Language | English |
| Publisher | Yonsei University College of Medicine |
| Publisher Date | 2005-10-01 |
| Access Restriction | Open |
| Rights License | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2005 The Yonsei University College of Medicine |
| Subject Keyword | Embryonic stem cells embryoid body differentiation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
