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Crystallization and preliminary X-ray crystallographic analysis of free methionine-(R)-sulfoxide reductase from Staphylococcus aureus.
| Content Provider | Europe PMC |
|---|---|
| Author | Bong, Seoung Min Moon, Jin Ho Kim, Hwa Young Kim, Hong Seok Chi, Young Min Kim, Augustine Yonghwi |
| Copyright Year | 2009 |
| Description | A hexagonal crystal of free methionine-(R)-sulfoxide reductase from S. aureus has been obtained using ammonium sulfate as a precipitant. Free methionine-(R)-sulfoxide reductase (fRMsr) catalyzes the reduction of the free form of methionine-(R)-sulfoxide back to free methionine. The fRMsr protein from Staphylococcus aureus was overexpressed in Escherichia coli, purified and crystallized at 295 K using ammonium sulfate as a precipitant. Diffraction data were collected to 1.7 Å resolution from a native crystal using synchrotron radiation. The crystal belonged to the hexagonal space group P6122, with unit-cell parameters a = b = 89.84, c = 88.75 Å, α = β = 90, γ = 120°. Assuming the presence of one molecule in the asymmetric unit, the calculated Matthews coefficient value was 2.21 Å3 Da−1, with a solvent content of 57.1%. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2777039&blobtype=pdf |
| Volume Number | 65 |
| PubMed Central reference number | PMC2777039 |
| Issue Number | Pt 11 |
| Issue Number | pt 11 |
| PubMed reference number | 19923731 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun] |
| e-ISSN | 17443091 |
| DOI | 10.1107/s1744309109037105 |
| Language | English |
| Publisher | International Union of Crystallography |
| Publisher Date | 2009-10-30 |
| Access Restriction | Open |
| Rights License | © International Union of Crystallography 2009 |
| Subject Keyword | methionine sulfoxide free methionine free methionine-(R)-sulfoxide reductase |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology |
