NDLI: Expressions of uroplakins in the mouse urinary bladder with cyclophosphamide-induced cystitis.

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Expressions of uroplakins in the mouse urinary bladder with cyclophosphamide-induced cystitis.

Content Provider	Europe PMC
Author	Choi, Seong Hoo Byun, Youngmin Lee, Gilho
Copyright Year	2009
Abstract	Even though uroplakins (UPs) are believed to serve a strong protective barrier against toxic materials, cyclophosphamide (CP) causes extensive cystitis. We investigated the expression of UPs in the urothelium in CP induced mouse cystitis. A total of 27 ICR female mice received a single intraperitoneal injection of 200 mg CP/kg. Nine CP-treated mice and 6 controls were sequentially killed at 12, 24, and 72 hr post injection. Extensive cystitis and an increased vesical weight were seen. These all peaked within 12 hr post injection and they tended to decrease thereafter. The level of all the UPs mRNA, the protein expressions of UP II and III on immunoblotting study, and the expression of UP III on immunolocalization study were maximally suppressed within 12 hr; this partially recovered at 24 hr, and this completely recovered at 72 hr post CP injection. In conclusion, CP reduced the expression of UPs. The reduction of the UPs mRNA and protein was time dependent, and this peaked within 12 hr after CP injection. However, the damage was rapidly repaired within 24 hr. This study demonstrates a dynamic process, an extensive reduction and rapid recovery, for the UPs expression of the mouse urinary bladder after CP injection.
ISSN	10118934
Volume Number	24
PubMed Central reference number	PMC2719198
Issue Number	4
PubMed reference number	19654953
Journal	Journal of Korean Medical Science [J. Korean Med. Sci]
e-ISSN	15986357
DOI	10.3346/jkms.2009.24.4.684
Language	English
Publisher	The Korean Academy of Medical Sciences
Publisher Date	2009-07-30
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2009 The Korean Academy of Medical Sciences
Subject Keyword	Cyclophosphamide Cystitis Uroplakin
Content Type	Text
Resource Type	Article
Subject	Medicine

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