NDLI: Estimation of 16S rRNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken.

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Estimation of 16S rRNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken.

Content Provider	Europe PMC
Author	Lee, Chin Mei Sieo, Chin Chin Abdullah, Norhani Ho, Yin Wan
Editor	Kneifel, Wolfgang
Copyright Year	2008
Abstract	The copy numbers of 16S rRNA genes in 12 probiotic Lactobacillus strains of poultry origin were analyzed. Genomic DNA of the strains was digested with restriction endonucleases that do not cut within the 16S rRNA gene of the strains. This was followed by Southern hybridization with a biotinylated probe complementary to the 16S rRNA gene. The copy number of the 16S rRNA gene within a Lactobacillus species was found to be conserved. From the hybridization results, Lactobacillus salivarius I 24 was estimated to have seven copies of the 16S rRNA gene, Lactobacillus panis C 17 to have five copies and Lactobacillus gallinarum strains I 16 and I 26 four copies. The 16S rRNA gene copy numbers of L. gallinarum and L. panis reported in the present study are the first record. Lactobacillus brevis strains I 12, I 23, I 25, I 211, I 218 and Lactobacillus reuteri strains C 1, C 10, C 16 were estimated to have at least four copies of the 16S rRNA gene. In addition, distinct rRNA restriction patterns which could discriminate the strains of L. reuteri and L. gallinarum were also detected. Information on 16S rRNA gene copy number is important for physiological, evolutionary and population studies of the bacteria.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2613232&blobtype=pdf
ISSN	03781097
Journal	FEMS Microbiology Letters [FEMS Microbiol Lett]
Volume Number	287
DOI	10.1111/j.1574-6968.2008.01305.x
PubMed Central reference number	PMC2613232
Issue Number	1
PubMed reference number	18707622
e-ISSN	15746968
Language	English
Publisher	Blackwell Publishing Ltd
Publisher Date	2008-08-14
Access Restriction	Open
Rights License	Reuse of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. © 2008 The Authors Journal compilation © 2008 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd
Subject Keyword	Lactobacillus spp. 16S rRNA gene copy number rRNA restriction patterns
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Microbiology

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