Generation of human monoclonal antibodies against Propionibacterium acnes by applying the phage display method to human peripheral blood mononuclear cells immunized in vitro.

Content Provider	Europe PMC
Author	Jung, Yeon Suk Matsumoto, Shin-Ei Katakura, Yoshinori Yamashita, Makiko Tomimatsu, Kosuke Kabayama, Shigeru Teruya, Kiichiro Shirahata, Sanetaka
Abstract	Propionibacterium acnes is a gram-positive, non-spore-forming, rod-shaped bacterium that is often detected in normal human skin flora. P. acnes has been associated with many diseases. In this study, we attempted to generate anti-P. acnes human monoclonal antibodies. A phage antibody library was first generated from human peripheral blood mononuclear cells immunized in vitro with P. acnes using the phage display method, and P. acnes-specific phage antibodies were obtained using solid phase panning. Antigen-specific variable region genes were then amplified and recombined into vectors expressing human IgG antibodies. The results indicated that the recombinant human IgG antibodies exhibited P. acnes-specific binding. This study demonstrates that the combined use of an in vitro immunization protocol and the phage display method enables the generation of human monoclonal antibodies against pathogenic bacteria and toxic antigens.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2553674&blobtype=pdf
ISSN	09209069
Volume Number	57
DOI	10.1007/s10616-008-9138-z
PubMed Central reference number	PMC2553674
Issue Number	2
PubMed reference number	19003162
Journal	Cytotechnology
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-02-22
Publisher Place	Dordrecht
Access Restriction	Open
Rights License	© Springer Science+Business Media B.V. 2008
Subject Keyword	Human monoclonal antibody In vitro immunization Phage display Propionibacterium acnes
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology