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Crystallization and preliminary X-ray diffraction analysis [correction of anaylsis] of the LOV1 domains of phototropin 1 and 2 from Arabidopsis thaliana.
| Content Provider | Europe PMC |
|---|---|
| Author | Nakasako, Masayoshi Hirata, Michihiro Shimizu, Nobutaka Hosokawa, Shyuntaro Matsuoka, Daisuke Oka, Toshihiko Yamamoto, Masaki Tokutomi, Satoru |
| Copyright Year | 2008 |
| Description | Crystals of the LOV1 domains of phototropin 1 and 2 from A. thaliana were obtained which diffracted X-rays to a resolution of at least 2.1 Å. Phototropin is a blue-light receptor protein in plants that is responsible for phototropic responses, stomata opening and photo-induced relocation of chloroplasts. Higher plants such as Arabidopsis thaliana have two isoforms of phototropin: phototropin 1 and phototropin 2. Both isoforms comprise a tandem pair of blue-light-absorbing light–oxygen–voltage domains named LOV1 and LOV2 in the N-terminal half and a serine/threonine kinase domain in the C-terminal half. The LOV1 domain is thought to function as a dimerization site. In the present study, recombinant LOV1 domains of A. thaliana phototropin 1 and phototropin 2 were crystallized. The crystal of the LOV1 domain of phototropin 1 belonged to the orthorhombic space group P212121, with unit-cell parameters a = 61.2, b = 64.9, c = 70.8 Å, and diffracted X-rays to a resolution of 2.1 Å. The crystal of the LOV1 domain of phototropin 2 belonged to space group P21, with unit-cell parameters a = 32.5, b = 66.5, c = 56.7 Å, β = 92.4°, and diffracted X-rays to beyond 2.0 Å resolution. In both crystals, two LOV1 domains occupied the crystallographic asymmetric unit. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2443956&blobtype=pdf |
| Volume Number | 64 |
| PubMed Central reference number | PMC2443956 |
| Issue Number | Pt 7 |
| Issue Number | pt 7 |
| PubMed reference number | 18607090 |
| Journal | Acta Crystallogr Sect F Struct Biol Cryst Commun |
| e-ISSN | 17443091 |
| DOI | 10.1107/s1744309108015534 |
| Language | English |
| Publisher | International Union of Crystallography |
| Publisher Date | 2008-06-11 |
| Access Restriction | Open |
| Rights License | © International Union of Crystallography 2008 |
| Subject Keyword | phototropins LOV domains blue-light receptors |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology |
