NDLI: Expression, purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of metabotropic glutamate receptor 7.

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Expression, purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of metabotropic glutamate receptor 7.

Content Provider	Europe PMC
Author	Muto, Takanori Tsuchiya, Daisuke Morikawa, Kosuke Jingami, Hisato
Copyright Year	2007
Description	The ligand-binding domain of metabotropic glutamate receptor 7 has been overexpressed, purified, and crystallized by the hanging-drop vapour-diffusion method. A complete data set has been collected to 3.30 Å. Glutamate is the major excitatory neurotransmitter and its metabotropic glutamate receptor (mGluR) plays an important role in the central nervous system. The ligand-binding domain (LBD) of mGluR subtype 7 (mGluR7) was produced using the baculovirus expression system and purified from the culture medium. The purified protein was characterized by gel-filtration chromatography, SDS–PAGE and a ligand-binding assay. Crystals of mGluR7 LBD were grown at 293 K by the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 3.30 Å resolution using synchrotron radiation and belong to the trigonal space group P3121, with unit-cell parameters a = b = 92.4, c = 114.3 Å. Assuming the presence of one protomer per crystallographic asymmetric unit, the Matthews coefficient V M was calculated to be 2.5 Å3 Da−1 and the solvent content was 51%.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2335145&blobtype=pdf
Volume Number	63
PubMed Central reference number	PMC2335145
Issue Number	Pt 7
Issue Number	pt 7
PubMed reference number	17620729
Journal	Acta Crystallogr Sect F Struct Biol Cryst Commun
e-ISSN	17443091
DOI	10.1107/s1744309107030059
Language	English
Publisher	International Union of Crystallography
Publisher Date	2007-06-29
Access Restriction	Open
Rights License	© International Union of Crystallography 2007
Subject Keyword	metabotropic glutamate receptors ligand-binding domains
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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