NDLI: Crystallization and preliminary X-ray crystallographic analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Mycobacterium tuberculosis.

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Crystallization and preliminary X-ray crystallographic analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Mycobacterium tuberculosis.

Content Provider	Europe PMC
Author	Webby, Celia J. Lott, J. Shaun Baker, Heather M. Baker, Edward N. Parker, Emily J.
Copyright Year	2005
Description	Crystals of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from M. tuberculosis have been grown and a native data set has been collected to a maximum resolution of 2.5 Å. The enzymes of the shikimate pathway are attractive targets for new-generation antimicrobial agents. The first step of this pathway is catalysed by 3-deoxy-d-arabino-heptulosonate-7-phosphate (DAH7P) synthase and involves the condensation of phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P) to form DAH7P. DAH7P synthases have been classified into two apparently evolutionarily unrelated types and whereas structural data have been obtained for the type I DAH7P synthases, no structural information is available for their type II counterparts. The type II DAH7P synthase from Mycobacterium tuberculosis was co-expressed as native and selenomethionine-substituted protein with the Escherichia coli chaperonins GroEL and GroES in E. coli, purified and crystallized. Native crystals of M. tuberculosis DAH7P synthase belong to space group P3121 or P3221 and diffract to 2.5 Å, with unit-cell parameters a = b = 203.61, c = 66.39 Å. There are either two or three molecules in the asymmetric unit. Multiwavelength anomalous diffraction (MAD) phasing using selenomethionine-substituted protein is currently under way.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC1952427&blobtype=pdf
Volume Number	61
PubMed Central reference number	PMC1952427
Issue Number	Pt 4
Issue Number	pt 4
PubMed reference number	16511053
Journal	Acta Crystallogr Sect F Struct Biol Cryst Commun
e-ISSN	17443091
DOI	10.1107/s1744309105007931
Language	English
Publisher	International Union of Crystallography
Publisher Date	2005-03-24
Access Restriction	Open
Rights License	© International Union of Crystallography 2005
Subject Keyword	Mycobacterium tuberculosis shikimate pathway chorismate biosynthesis 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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