Molecular Fingerprinting of Mouse Brain Using Ultrabroadband Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy Empowered by Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS).

Content Provider	Europe PMC
Author	Murakami, Yusuke Ando, Masahiro Imamura, Ayako Oketani, Ryosuke Leproux, Philippe Honjoh, Sakiko Kano, Hideaki
Copyright Year	2024
Description	The Raman fingerprint spectral region provides abundant structural information on molecules. However, analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Raman cross section and congested spectral features. In this study, we combined ultrabroadband coherent anti-Stokes Raman scattering (CARS) microspectroscopy across the spectral range of 500–4000 cm–1 with multivariate curve resolution-alternating least-squares (MCR-ALS) to reveal hidden Raman bands in the fingerprint region. Applying this method to mouse brain tissue, we extracted information on cholesterol and collagen, leveraging their distinctive molecular signatures, as well as on key molecules such as lipids, proteins, water, and nucleic acids. Moreover, the simultaneous detection of second harmonic generation facilitated label-free visualization of organelles, including arachnoid membrane and Rootletin filaments.
Abstract	The Raman fingerprint spectral regionprovides abundant structuralinformation on molecules. However, analyzing vibrational images withinthis region using coherent Raman imaging remains challenging due tothe small Raman cross section and congested spectral features. Inthis study, we combined ultrabroadband coherent anti-Stokes Ramanscattering (CARS) microspectroscopy across the spectral range of 500–4000cm–1 with multivariate curve resolution-alternatingleast-squares (MCR-ALS) to reveal hidden Raman bands in the fingerprintregion. Applying this method to mouse brain tissue, we extracted informationon cholesterol and collagen, leveraging their distinctive molecularsignatures, as well as on key molecules such as lipids, proteins,water, and nucleic acids. Moreover, the simultaneous detection ofsecond harmonic generation facilitated label-free visualization oforganelles, including arachnoid membrane and Rootletin filaments.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC11522994&blobtype=pdf
Volume Number	2
DOI	10.1021/cbmi.4c00034
PubMed Central reference number	PMC11522994
Issue Number	10
PubMed reference number	39483635
Journal	Chemical & Biomedical Imaging [Chem Biomed Imaging]
e-ISSN	28323637
Language	English
Publisher	Nanjing University and American Chemical Society
Publisher Date	2024-07-25
Access Restriction	Open
Rights License	Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). © 2024 The Authors. Co-published by Nanjing University and American Chemical Society
Subject Keyword	molecular fingerprinting coherent anti-Stokes Raman scattering CARS microscopy multivariate curve resolution-alternating least-squares MCR-ALS brain
Content Type	Text
Resource Type	Article
Subject	Medicine