Crystal Structure of Bifidobacterium bifidum Glycoside Hydrolase Family 110 α-Galactosidase Specific for Blood Group B Antigen.

Content Provider	Europe PMC
Author	Kashima, Toma Akama, Megumi Wakinaka, Takura Arakawa, Takatoshi Ashida, Hisashi Fushinobu, Shinya
Abstract	To overcome incompatibility issues and increase the possibility of blood transfusion, technologies that enable efficient conversion of A- and B-type red blood cells to the universal donor O-type is desirable. Although several blood type-converting enzymes have been identified, detailed understanding about their molecular functions is limited. α-Galactosidase from Bifidobacterium bifidum JCM 1254 (AgaBb), belonging to glycoside hydrolase (GH) 110 subfamily A, specifically acts on blood group B antigen. Here we present the crystal structure of AgaBb, including the catalytic GH110 domain and part of the C-terminal uncharacterized regions. Based on this structure, we deduced a possible binding mechanism of blood group B antigen to the active site. Site-directed mutagenesis confirmed that R270 and E380 recognize the fucose moiety in the B antigen. Thermal shift assay revealed that the C-terminal uncharacterized region significantly contributes to protein stability. This region is shared only among GH110 enzymes from B. bifidum and some Ruminococcus species. The elucidation of the molecular basis for the specific recognition of blood group B antigen is expected to lead to the practical application of blood group conversion enzymes in the future.
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ISSN	13447882
Volume Number	71
PubMed Central reference number	PMC11368712
Issue Number	3
PubMed reference number	39234034
Journal	Journal of Applied Glycoscience [J Appl Glycosci (1999)]
e-ISSN	18807291
DOI	10.5458/jag.jag.jag-2024_0005
Language	English
Publisher	The Japanese Society of Applied Glycoscience
Publisher Date	2024-08-20
Publisher Place	Tokyo, JAPAN
Access Restriction	Open
Rights License	This is an open-access paper distributed under the terms of the Creative Commons Attribution Non-Commercial (by-nc) License (CC-BY-NC4.0: https://creativecommons.org/licenses/by-nc/4.0/). 2024 by The Japanese Society of Applied Glycoscience
Subject Keyword	Blood group conversion crystal structure glycoside hydrolase Bifidobacterium mucin α-galactosidase
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology