NDLI: Identification and Characterization of Novel Intracellular α-Xylosidase in <i>Aspergillus oryzae</i>.

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Identification and Characterization of Novel Intracellular α-Xylosidase in Aspergillus oryzae.

Content Provider	Europe PMC
Author	Matsuzawa, Tomohiko Nakamichi, Yusuke Shimada, Naoki
Abstract	α-Xylosidase releases xylopyranosyl side chains from xyloglucan oligosaccharides and is vital for xyloglucan degradation. Previously, we identified and characterized two α-xylosidases, intracellular AxyA and extracellular AxyB, in Aspergillus oryzae. In this study, we identified a third α-xylosidase, termed AxyC, in A. oryzae. These three A. oryzae α-xylosidases belong to the glycoside hydrolase family 31, but there are clear differences in substrate specificity. Both AxyA and AxyB showed much higher hydrolytic activity toward isoprimeverose (α-D-xylopyranosyl-1,6-glucose) than p-nitrophenyl α-D-xylopyranoside. In contrast, the specific activity of AxyC toward the p-nitrophenyl substrate was approximately 950-fold higher than that toward isoprimeverose. Our study revealed that there are multiple α-xylosidases with different substrate specificities in A. oryzae.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10792220&blobtype=pdf
ISSN	13447882
Volume Number	70
PubMed Central reference number	PMC10792220
Issue Number	4
PubMed reference number	38239767
Journal	Journal of Applied Glycoscience [J Appl Glycosci (1999)]
e-ISSN	18807291
DOI	10.5458/jag.jag.jag-2023_0007
Language	English
Publisher	The Japanese Society of Applied Glycoscience
Publisher Date	2023-12-20
Publisher Place	Tokyo, JAPAN
Access Restriction	Open
Rights License	This is an open-access paper distributed under the terms of the Creative Commons Attribution Non-Commercial (by-nc) License (CC-BY-NC4.0: https://creativecommons.org/licenses/by-nc/4.0/). 2023 by The Japanese Society of Applied Glycoscience
Subject Keyword	Aspergillus oryzae α-xylosidase glycoside hydrolase family 31 xyloglucan
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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