LC-MS-based serum metabolomics analysis for the screening and monitoring of colorectal cancer.

Content Provider	Europe PMC
Author	Yi, Yanan Wang, Jianjian Liang, Chengtong Ren, Chuanli Lian, Xu Han, Chongxu Sun, Wei
Copyright Year	2023
Abstract	BackgroundColorectal Cancer (CRC) is a prevalent digestive system tumour with significant mortality and recurrence rates. Serum metabolomics, with its high sensitivity and high throughput, has shown potential as a tool to discover biomarkers for clinical screening and monitoring of the CRC patients.MethodsSerum metabolites of 61 sex and age-matched healthy controls and 62 CRC patients (before and after surgical intervention) were analyzed using a ultra-performance liquid chromatography-high resolution mass spectrometer (UPLC-MS). Statistical methods and pathway enrichment analysis were used to identify potential biomarkers and altered metabolic pathways.ResultsOur analysis revealed a clear distinction in the serum metabolic profile between CRC patients and healthy controls (HCs). Pathway analysis indicated a significant association with arginine biosynthesis, pyrimidine metabolism, pantothenate, and CoA biosynthesis. Univariate and multivariate statistical analysis showed that 9 metabolites had significant diagnostic value for CRC, among them, Guanosine with Area Under the Curve (AUC) values of 0.951 for the training group and0.998 for the validation group. Furthermore, analysis of four specific metabolites (N-Phenylacetylasparticacid, Tyrosyl-Gamma-glutamate, Tyr-Ser and Sphingosine) in serum samples of CRC patients before and after surgery indicated a return to healthy levels after an intervention.ConclusionOur results suggest that serum metabolomics may be a valuable tool for the screening and monitoring of CRC patients.
Page Count	11
ISSN	2234943X
Journal	Frontiers in Oncology
Volume Number	13
PubMed Central reference number	PMC10338013
PubMed reference number	37448516
e-ISSN	2234943X
DOI	10.3389/fonc.2023.1173424
Language	English
Publisher	Frontiers Media S.A.
Publisher Date	2023-06-28
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Copyright © 2023 Yi, Wang, Liang, Ren, Lian, Han and Sun
Subject Keyword	colorectal cancer serum metabolomics liquid chromatography-mass spectrometer biomarkers screening monitoring
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology