NDLI: GDSC SMLM: Single-molecule localisation microscopy software for ImageJ.

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GDSC SMLM: Single-molecule localisation microscopy software for ImageJ.

Content Provider	Europe PMC
Author	Etheridge, Thomas J. Carr, Antony M. Herbert, Alex D.
Copyright Year	2022
Abstract	Single-molecule localisation microscopy (SMLM) uses software to extract super-resolved positions from microscope images of fluorescent molecules. These localisations can then be used to render super-resolution images or analysed to extract information about molecular behaviour. The GDSC SMLM software provides a set of tools for analysing SMLM data in a single cross-platform environment. The software identifies fluorescent molecules in raw microscope images and localises their positions using stages of spot detection, spot fitting and spot rejection. The resulting localisation data set can then be visualised, cropped and filtered. A suite of downstream analysis tools enable the user to perform single-particle tracking, cluster analysis and drift correction. In addition, GDSC SMLM also provides utility tools that enable modelling of EM-CCD and sCMOS cameras as well as point spread functions (PSFs) for data simulation. The software is written in Java and runs as a collection of plugins for the ImageJ software.
Journal	Wellcome Open Research
Volume Number	7
PubMed Central reference number	PMC10282564
PubMed reference number	37351368
e-ISSN	2398502X
DOI	10.12688/wellcomeopenres.18327.1
Language	English
Publisher	F1000 Research Limited
Publisher Date	2022-09-29
Publisher Place	London, UK
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright: © 2022 Etheridge TJ et al.
Subject Keyword	SMLM single-molecule super-resolution ImageJ Java fluorescence imaging software
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology

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